Hemp Testing & CBD Lab Testing

We Test and Certify Extracts, Flower, and Products

ACS Laboratory team grouped up around a table

Testing Hemp Is at the Core
of Everything We Do

At ACS Laboratory, it is our purpose to instill confidence and assure product quality by providing the most comprehensive and precise analytical testing services and solutions for our clients in the hemp business, from hemp cultivators, to extractors, manufacturers, wholesalers, retailers, as well as those in the industrial hemp and finished CBD product spaces.
Cannabinoid Potency
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Cannabinoid Potency
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Cannabis potency testing measures the cannabinoids present in your cannabis sample and measures the strength of those compounds. ACS Laboratory uses an Ultra High-Performance Liquid Chromatography (HPLC), which is the preferred instrument for testing cannabis edibles and extracts due to its ability to test samples at room temperature without requiring a catalyst to produce a heat reaction. This method can also test for THC Acid (THCA) and CBD Acid (CBDA), which a gas chromatograph would not.

Matrix

Flowers/Plants, Derivative Productss and Edibles

Analytes

Up to 25 Count

Instrument

LCMS

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Terpene Profiling
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Terpene Profiling
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Terpenes are produced in trichromes (where THC is produced) and give cannabis its distinctive flavor and aroma. Terpenes also act as essential, medicinal hydrocarbon building blocks, influencing the overall homeopathic effect. From the pine odor of pinene to the citrus-like smell of limonene, the characterization of terpenes and their synergistic effect with cannabinoids is reliably identified in the spectral library of terpene analysis to meet terpene profiling needs.

Matrix

Flowers / Biomass /
Derivatives / Edibles

Analytes

38 Count

Instrument

GCMS/GCFID
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(+)-Cedrol
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3-Carene
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Camphor
(mix of isomers)
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Caryophyllene oxide
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Cis Nerolidol
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Fenchone
(mix of isomers)
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Gamma-Terpinene
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Gamma-Terpineol
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Geraniol
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Geranyl Acetate
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Hexahydrothymol
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Isoborneol
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Isopulegol
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Nerol(cis Geraniol)
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Pulegone
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Sabinene
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Sabinene Hydrate
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Terpinolene
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Trans-Caryophyllene
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Trans-Nerolidol
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alpha-Cedrene
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alpha-Terpinene
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alpha-Terpineol
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beta-Pinene

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Flavonoids Testing
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Flavonoids Testing
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Flavonoids are phytonutrients most important for plant pigments, flower coloration, taste, smell and overall sensory experience. They are a high level of nutrients and are found in many common foods consumed today. They contribute to the growth of cannabis as well as provide other health advantages and an overall sensory experience.

Matrix

Flowers/Plants, Derivative Products and Edibles

Analytes

16 Count

Instrument

LCMS/MS

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Moisture Activity
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Moisture Activity
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Hemp and cannabis plants need water to grow and thrive, and yet your final products must be stripped of most of their moisture in order to guarantee safety. Overmoisturization can occur as a result of overwatering, high humidity or an ineffective curing process. This is especially a risk for plants that grow outside, which are more susceptible to environmental conditions. You’ll need to test your post-harvest hemp for water activity and moisture content because these are key factors that can indicate risks for microbial contamination.

Matrix

Flowers/Plants, Derivative Products and Edibles

Analytes

1 Count

Instrument

MOISTURE ANALYZER
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Moisture

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Pesticides
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Pesticides
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ACS offers the most sensitive and comprehensive pesticide screening and confirmation available utilizing  Gas Chromatography - Tandem Mass Spectrometry (GCMS) and Ultra High-Performance Liquid Chromatography (HPLC) Tandem Mass Spectrometry (LCMS).

Pesticides Testing

Matrix

Flowers / Biomass / Derivatives / Edibles

Analytes

68 - 66 -102 Count

Instrument

GCMS / MS* and
LCMS / MS

Florida Guidelines 68

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Abamectin
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Acephate
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Acequinocyl
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Acetamiprid
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Aldicarb
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Azoxystrobin
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Bifenazate
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Bifenthrin
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Boscalid
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Captan*
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Carbaryl
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Carbofuran
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Chlorantraniliprole
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Chlordane*
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Chlorfenapyr*
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Chlormequat Chloride
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Chlorpyrifos
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Clofentezine
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Coumaphos*
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Utah Guidelines 66

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Cyfluthrin*
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Cypermethrin
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Daminozide
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Diazinon
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Dichlorvos
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Dimethoate
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Dimethomorph*
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Ethoprop(hos)
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Etofenprox
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Colorado Guidelines 102

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Etofenprox
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Etoxazole
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Etridiazole
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Fenhexamid
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Fenoxycarb
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Fenthion
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Fenvalerate
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Fipronil
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Flonicamid
+ 91 more

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Residual Solvents
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Residual Solvents
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Solvent-based extraction produces high-quality concentrates using chemicals like butane, propane, and ethanol. To ensure these potent, terpene-rich formulas are safe to ingest, ACS verifies your product is compliant with all residual solvent limits using Gas Chromatography - Mass Spectrometry (GC-MS).

Matrix

Flowers/Plants, Derivative Products and Edibles

Analytes

21 - 41 - 1 Count

Instrument

GCMS

Florida

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1,2 Dimethoxyethane
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1,4 Dioxane
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1-Butanol
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1-Pentanol
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1-Propanol
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2,2-dimethylbutane
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2,3-dimethylbutane
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2-Butanol
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2-Butanone
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Utah

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2-Ethoxyethanol
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2-Methylbutane
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2-Methylpentane
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2-Propanol (IPA)
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3-Methylpentane
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Acetone
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Acetonitrile
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Benzene
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Butane
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2,3 Butanedione

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2,3-butanedione

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Microbiology
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Microbiology
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Similar conditions for growth of cannabis are ideal for the germination of microorganisms and bacteria. If cannabis is not tested properly, it may contain contaminants that are harmful for human consumption. These contaminants can lead to serious illness and health complications. Whether a client wants to test cannabis by traditional plate count growth or by qPCR (Real Time Polymerase Chain Reaction) technology or by Ultra HPLC MS/MS, ACS provides accurate results of quality that the end user can depend on.

MICROARRAY available for Edibles and Extracts. qPCR TECHNOLOGY available for Flowers and Biomass (flower/mulch). If qPCR is positive then test for Microarray.

Pathogenic (Hemp Compliance Panel)

Matrix

Flowers, Plants, Derivative Products and Edibles

Analytes

7 Count

Instrument

qPCR Technology
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Aspergillus Flavus
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Aspergillus Fumigatus
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Aspergillus Niger
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Aspergillus Terreus
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Salmonella species
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Shiga Toxin Prod. E. Coli (STEC)
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Listeria Monocytogenes

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Heavy Metals Screening
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Heavy Metals Screening
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Cannabis collects heavy metals. It absorbs heavy metals from the soil, water and air. ICPMS (Inductively Coupled Plasma Mass Spectrometry) testing is the gold standard. These toxic elements can be passed from the plant to the consumer and pose serious health risks. Screening for heavy metals ensures the end user will have a safe and confident product. ACS can detect heavy metals at a very low level of concentration, ensuring safety to the end user.

Matrix

Flowers / Biomass /
Derivatives / Edibles

Analytes

9 Count

Instrument

ICPMS
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Arsenic
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Cadmium
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Lead
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Mercury
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Palladium
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Platinum
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Nickel
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Zinc
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Manganese

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Beverage Testing
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Beverage Testing
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Nano emulsions are the latest innovation in cannabis beverages that utilize “water soluble cannabinoids.” In nano emulsions, THC, CBD and other cannabinoids are broken down to such small sizes that they become emulsified in water and remain intact, without separating. Because of their microscopic particle size and relatively low cannabinoid concentration, nano beverages are the hardest to test. Fortunately, ACS Laboratory is one of only 4 labs in the United States that won the Emerald Test Badge award for testing beverage potency.

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Theanine-Caffeine - Melatonin
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Theanine-Caffeine - Melatonin
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Matrix

Flowers/Plants, Derivative Products and Edibles

Analytes

27 Count

Instrument

ICPMS
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L-theanine
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Caffeine
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Melatonin

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pH Level
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pH Level
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Matrix

Derivative Products and Edibles

Analytes

1 Count

Instrument

pH Meter
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pH Level

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Hemp Sample Collection
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Hemp Sample Collection
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The Florida Department of Agriculture and Consumer Services (FDACS) does not allow farmers to clip their own samples for testing. Instead, they must enlist approved third-party laboratories to conduct this task. Through ACS’s designation as the largest DEA-registered hemp testing laboratory in Florida, we can collect hemp samples, transport them to our facility, and offer a full suite of hemp analyses including the mandated THC potency requirement. We also test for common contaminants, including pesticides, heavy metals, molds, and bacteria.

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Vitamin E Acetate
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Vitamin E Acetate
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In vapes, vitamin E acetate is a cheap thinning agent used to dilute or thicken the THC or CBD substance, which enhances its usage in cartridges. As of the latest health outbreak, it is considered a major contaminant to your product and a threat to customer safety. Your customers want to be sure that their vape product is 100% vitamin E acetate-free.

Matrix

Vape Oils

Analytes

1 Count

Instrument

LCMS
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Tocopheryl Acetate

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Triple Virus Testing
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Triple Virus Testing
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Matrix

Flowers

Analytes

3 Count

Instrument

qPCR
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Cannabis Cryptic Virus
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Hops Latent Viroid
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Lettuce Chlorosis Virus

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Mycotoxins
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Mycotoxins
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Mycotoxins are contaminants that naturally occur in molds and fungi, which decompose organic matter. You can find molds that contain mycotoxins in a variety of crops such as nuts, spices, apples and coffee beans. You can also find mycotoxins in cannabis flower and its derivative products such as oils, edibles, tinctures and creams. Your hemp or cannabis products are especially at risk for mycotoxins in warm and humid environments.
Mycotoxins can be very harmful to humans, especially in cases of long-term exposure, so you want to be sure that your products are safe for use. Regardless of how carefully you produce and store your cannabis, or how much you trust your business partner’s cultivation methods, you can never be sure that your product is safe until you test with an accredited laboratory like ACS.

Matrix

Flowers/Plants, Derivative Products and Edibles

Analytes

5 Count

Instrument

LCMS
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Aflatoxins B1
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Aflatoxins B2
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Aflatoxins G1
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Aflatoxins G2
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Ochratoxin A

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Shelf Life and Stability Study
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Shelf Life and Stability Study
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Customers shop for brands whose products they trust. The products should contain high-quality ingredients that match the label and are safe to consume. Like all consumable goods, cannabis and hemp quality can degrade over time due to conditions such as temperature, lighting, air and humidity. Through shelf life and stability testing, you can discover how environmental conditions may affect your product’s potency, contamination, color, odor and moisture.

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More About Shelf Life Stability

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Transdermal Patches
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Transdermal Patches
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Transdermal Patches are growing in popularity as a preferred alternative to smoking or ingesting, due to their superior dosing control, slower release and increased bioavailability. This is especially true for patients who do not want to smoke or ingest the products. That’s why it’s so important for brands to understand exactly what concentration of healing ingredients their transdermal patches contain.

Matrix

Flowers, Plants, Derivative Products and Edibles

Analytes

Varies

Instrument

qPCR Technology

Cannabinoid Potency

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CBD
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CBDA
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CBDV
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CBG
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CBGA
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Δ9-THC
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Δ8-THC
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THCA-A
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THCV
+ 14 more

Terpene Potency

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Limonene
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Linalool
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Humulene
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Pinene
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Myrcene
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Guaiol
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Ocimene
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Bisabolol
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Nerol
+ 29 more

Flavonoid Potency

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Apigenin
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Baicalin
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Beta Sitosterol
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Cannflavin A
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Cannflavin B
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Cannflavin C
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Chrysin
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Fisetin
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Kaempferol
+ 7 more

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Edibles
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Edibles
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Our methods overcome the challenges of edibles testing and guarantee precision. Before testing edibles for potency, we utilize what’s known as a cryogenic grinder or freezer/mill, where we flash-freeze and pulverize the edible to homogenize it. This method provides consistent, reliable results, and we have the awards to prove it. For the last 4 years, we have received badges for excellence in edibles potency from the Emerald Test, a nationwide Inter-Laboratory Comparison test.

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More About Edibles

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Pre-Harvest Test
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Pre-Harvest Test
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To comply with legal requirements, you must select seeds from certified suppliers with low THC genetics. However, your harvested flower won’t always match the seed’s expected genetic profile. This can lead to accidentally growing hot hemp.That’s why ACS tests seedlings to estimate the THC potency; as well as for  potency of up to 30 other cannabinoids. Through vegetative potency tests, we can analyze plants early in their life cycles to help enable rapid, high quality development.

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More About Pre-Harvest

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Nano Testing
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Nano Testing
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ACS tests nanotechnology cannabis consumables with an award-winning methodology. Using Ultra High-Performance Liquid Chromatography (HPLC) with ultraviolet detection, we deliver reliable cannabinoid and terpene profiling. We currently test nano products for 30 common cannabinoids as well as 38 terpenes and 16 flavonoids to accurately profile the product’s flavor, fragrance and therapeutic effects.

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More About Nano Testing

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Topicals Testing
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Topicals Testing
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ACS is rated in the top 5% in the U.S. for hemp and cannabis testing. Our methods are proven and we guarantee precision. Before testing topicals for potency, we meticulously extract the delicate cannabinoids from the product. Then we determine potency through High-Performance Liquid Chromatography (HPLC) using a UV wavelength detector to identify various forms of cannabinoids. This method provides consistent, reliable results that you can only find with a top-rated lab like ACS.In the last 4 years we have received 82 Emerald Badges for excellence from the Emerald Test, a nationwide Inter-Laboratory Comparison test.

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More About Topicals testing

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Concentrates Testing
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Concentrates Testing
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ACS guarantees precise results when testing concentrates by utilizing proven methods known for accuracy. We use Ultra High-Performance Liquid Chromatography (HPLC) with Ultraviolet Detectors to safely test samples at room temperature without heat. This method provides consistent and reliable results, and we have the awards to prove it. For the last two years, we’ve received awards for excellence in potency testing from the Emerald Test, a nationwide Inter-Laboratory Comparison test.

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More About Concentrates Testing

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Vape Testing
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Vape Testing
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Our methods for vape testing guarantee precise results. Through our proven methods, we employ Ultra High-Performance Liquid Chromatography with Ultraviolet Detectors to safely test samples at room temperature without heat.

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Plant Health Panel
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Plant Health Panel
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ACS offers the most sensitive and comprehensive pesticide screening and confirmation available utilizing Ultra High-Performance Liquid Chromatography (HPLC)-Tandem Mass Spectrometry (MS/MS). Offering excellent sensitivity and throughput, the ultra-low detection limits provided by ACS’s proprietary methodology make this technique ideal for the analysis of pesticides commonly employed during cannabis cultivation." to lower on the page after Viruses before Pestices.

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More About Plant Health Panel

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Our Credentials

Emerald Scientific logo without text in dark green
Emerald Proficiency Test
US Drug Enforcement Administration logo
Drug Enforcement Administration
DEA Registered Laboratory
#RA0571996

Clinical Laboratory Improvement Amendments logo.
CLIA Certified
Agency for Health Care
Administration

Work with Florida’s
largest in state
designated lab

ACS Laboratory is ISO/IEC 17025:2017 Accredited by A2LA #6786.01, DEA registered (#RA0571996), and CLIA licensed with the largest state-of-the-art facility in the eastern USA. Compliant with the USDA’s rules for hemp testing, ACS is also approved by the Florida Department of Agriculture as a “Designated Compliance Laboratory,” and deemed a "Certified Marijuana Testing Laboratory"  by the Florida Department of Health. Due to its success, ACS Laboratory has undergone a 20,000 sq. ft. expansion and increased its reach to 50 states and Puerto Rico as well as 16 countries worldwide.

With the exponential and rapid increase of hemp production over the past decade comes a class of consumers that are more informed about how to use various metrics and specifications, such as the certificates of analysis that we provide for our client's products, to discern premium from mediocre quality products.

We are fully equipped to test every type of hemp product for our clients, including biomass, flower, extracts, nano emulsions, beverages, topicals, creams, concentrates, edibles, vapes, and tinctures.

What We Test For

While myriad cultivators are concentrated on producing hemp containing less than .3% THC, a recent trend is emerging wherein many cannabis companies are beginning to focus on data related to minor cannabinoids, such as CBG, CBT, THCV, CBDVA and a plethora of others. Along with testing for cannabinoids, we emphasize our ability to quantitatively test for the highest number of terpenes and flavonoids in the United States.
At this point, the majority of individuals in the cannabis industry have heard about terpenes and the importance of them, but not everyone fully comprehends their relevancy and mechanism of action. Terpenes modulate the cannabinoid experience and are responsible for most of the nuance that is felt between different cultivars and samples, as well as provide a majority of the taste and aroma of cannabis. Essentially, they are supplemental to THC breaking through the blood brain barrier by providing variation and subtlety to the cannabis experience. In addition to terpenes, flavonoids also account for a large portion of the flavor profile. Like terpenes, flavonoids exist in a variety of plants other than cannabis.

How
We Test

We’ve developed advanced methodologies to accurately test all hemp products, from biomass, to extracts to nano emulsions.

We offer R&D testing from soil to oil. We also provide state-level and nationwide hemp compliance panels so you can confidently sell your products across the country. We utilize state-of-the-art equipment like Ultra High-Performance Liquid Chromatography (HPLC) to confirm our results.
Cannabis samples inside a test vial
A scientist transferring CBD oil over to a vial for potency testing
ACS Laboratory has won the most national awards and is a State Designated Laboratory that is registered with the DEA (#RA0571996)

Why it
Matters?

01.
Providing stellar customer service
02.
Exceeding industry standards
03.
Highly reproducible quality, results in a regulated environment
04.
Providing stellar customer service
Start Testing
As a hemp producer, it can be difficult to know how and when to test hemp raw materials and products. Producing an accurate hemp test entails obtaining a reliable sample through proper collection procedures for hemp and THC compliance, as outlined by the U.S. Department of Agriculture, in an effort to avoid any contamination. We also provide soil testing timelines, from testing the soil prior to planting and plants before harvest, to harvested flowers and finished concentrates.

In order to validate the results of every hemp sample report, a hemp certificate of analysis (COA), online results portal with a public view,  QR codes linked to COA for product packaging, and tracking via blockchain, the future of tamper-proofing.
An illustration of a microscope in green
When selecting a lab for testing hemp, there are a number of different metrics, accreditations, and testing methodologies that should be taken into account. First and foremost, as required by Drug Enforcement Administration regulations, our laboratory is registered with the DEA (#RA0571996). In addition, we are registered with the AHCA, licensed by CLIA and are ISO/IEC 17025:2017 Accredited by A2LA #6786.01, and have been awarded 82 Emerald Badges, an inter-laboratory comparison and proficiency test, in 2019, 21 in 2020, 19 in 2021 and 21 in 2022. As a result of our years of experience operating as a testing lab aiding in the quantitative aspects of medical research, ACS is able to disseminate clinical  grade results.

Beyond
Compliance

Start Testing
To ensure the most professional and trustworthy results, instead of outsourcing the lead scientist role, ACS has taken an important step in providing our own lead scientist. Additionally, we have invested in a 20,000 square ft site with all of the proper laboratory-grade instruments. Consequently, we are able to employ the most reliable testing methods available, such as high performance liquid chromatography mass spectrometry (HPLC/LCMS) and LCUV.
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Locker room of CBD experts with collection of lab coats on a rack
Group image of a chemical elements in violet
In 2014, under the Hemp Pilot Program, government regulations for hemp cultivation and hemp testing were first established, though this hemp program was quite limited in scope, scale, and applications. Subsequently in 2018, the USDA Farm Bill was passed to legalize and regulate broader cultivation of hemp, as well as specifically and explicitly differentiating hemp plants from cannabis plants. In order for a sample to qualify as "hemp," and thus qualify for the exemption from Schedule 1 status, the product must not exceed Delta-9-THC levels of .3%, according to current United States government regulations. Therefore any extract, derivative, or product with more than that limit is considered to be a controlled substance. However, there is a significant momentum developing in the cannabis industry behind increasing that potency limit to 1% in the United States, as it is in most of the world. In August of 2020, the DEA's Interim Final Rule was released.

Specifically, the Final Interim Rule elucidates the DEA's assertion that hemp processors can convert legal use hemp products into illegal use marijuana products, therefore bringing them under the DEA’s jurisdiction if said processing and extraction increases the THC concentration above the 0.3% threshold, even if it is for a transient moment during the extraction process.